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Imaging moving targets through scattering media

机译:通过散射介质成像移动目标

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摘要

Optical microscopy in complex, inhomogeneous media is challenging due to the presence of multiply scattered light that limits the depths at which diffraction-limited resolution can be achieved. One way to circumvent the degradation in resolution is to use speckle- correlation-based imaging (SCI) techniques, which permit imaging of objects inside scattering media at diffraction-limited resolution. However, SCI methods are currently limited to imaging sparsely tagged objects in a dark-field scenario. In this work, we demonstrate the ability to image hidden, moving objects in a bright-field scenario. By using a deterministic phase modulator to generate a spatially incoherent light source, the background contribution can be kept constant between acquisitions and subtracted out. In this way, the signal arising from the object can be isolated, and the object can be reconstructed with high fidelity. With the ability to effectively isolate the object signal, our work is not limited to imaging bright objects in the dark-field case, but also works in bright-field scenarios, with non-emitting objects.
机译:由于存在多重散射光,因此限制了可达到衍射极限分辨率的深度,因此在复杂,不均匀介质中的光学显微镜具有挑战性。避免分辨率降低的一种方法是使用基于斑点相关的成像(SCI)技术,该技术允许以衍射极限分辨率对散射介质内部的对象进行成像。但是,SCI方法当前仅限于在暗视场中对稀疏标记的对象进行成像。在这项工作中,我们演示了在明亮视野中对隐藏的移动物体成像的功能。通过使用确定性相位调制器生成空间上不相干的光源,可以在两次采集之间将背景贡献保持恒定并相减。这样,可以隔离从物体产生的信号,并且可以以高保真度重建物体。具有有效隔离对象信号的能力,我们的工作不仅限于在暗视场中对明亮的物体成像,而且还适用于具有非发光物体的明场场景。

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